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Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement Anais da ABC (AABC)
SANTOS,DALILHIA N. DOS; FERREIRA,JULIANO L.; SETOTAW,TESFAHUN A.; CANÇADO,GERALDO M.A.; PASQUAL,MOACIR; LONDE,LUCIANA C.N.; SATURNINO,HELOISA M.; VENDRAME,WAGNER A..
ABSTRACT Jatropha is a potential oilseed crop, which requires mitigating factors such as the low genetic variability of the species. The solution runs through the research of Brazilian germplasm. Attention should be given to the germplasm of jatropha the north of Minas Gerais, because this is the oldest national collection and because this region may be a regions of jatropha diversity due to selection pressure arising from environmental adversities. Therefore, the objective of this study was to investigate the genetic diversity of 48 accessions of collection from Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), using SSR and ISSR markers. The results showed low genetic diversity, but some individuals stood out as J. mollissima (48), J. podagrica...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Jatropha curcas; Molecular markers; Oilseed crop.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602363
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Genetic structure of annual ryegrass (Lolium multiflorum) populations estimated by RAPD Scientia Agricola
Vieira,Eduardo Alano; Castro,Caroline Marques; Oliveira,Antônio Costa de; Carvalho,Fernando Irajá Félix de; Zimmer,Paulo Dejalma; Martins,Luís Fernando.
Annual ryegrass is a temperate climate annual foraging grass, grown mostly in the South of Brazil, especially in the state of Rio Grande do Sul. Despite its importance, little is known about its genetic diversity, both within and among the populations cultivated. This knowledge is of fundamental importance for developing breeding and conservation strategies. The aim of this study was to characterize the genetic diversity and structure of four populations of annual ryegrass. Three of the populations were located in Rio Grande do Sul and the fourth in Uruguay. RAPD markers were used to study the genetic diversity and structure of these populations. Analysis of 375 individuals sampled from the populations, using six RAPD primers, generated a total of 82...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Foraging; Genetic diversity; Molecular markers.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400009
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Genetic structure of Argentinean hexaploid wheat germplasm Genet. Mol. Biol.
Vanzetti,Leonardo S.; Yerkovich,Nadia; Chialvo,Eugenia; Lombardo,Lucio; Vaschetto,Luis; Helguera,Marcelo.
The identification of genetically homogeneous groups of individuals is an ancient issue in population genetics and in the case of crops like wheat, it can be valuable information for breeding programs, genetic mapping and germplasm resources. In this work we determined the genetic structure of a set of 102 Argentinean bread wheat (Triticum aestivum L.) elite cultivars using 38 biochemical and molecular markers (functional, closely linked to genes and neutral ones) distributed throughout 18 wheat chromosomes. Genetic relationships among these lines were examined using model-based clustering methods. In the analysis three subpopulations were identified which correspond largely to the origin of the germplasm used by the main breeding programs in Argentina.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat; Genetic structure; Molecular markers; Genetic diversity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300014
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Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil Planta Daninha
Rocha,D.C.; Rodella,R.A.; Marino,C.L.; Martins,D..
This work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotype; Commelinaceae; Molecular markers; Weeds.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582009000300001
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Genetic variability for waxy genes in Argentinean bread wheat germplasm Electron. J. Biotechnol.
Vanzetti,Leonardo Sebastián; Pflüger,Laura Alicia; Rodríguez-Quijano,Marta; Carrillo,José Maria; Helguera,Marcelo.
Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with the lack of waxy protein(s). In this work, different sets of PCR markers were used to characterize the genetic variability of waxy loci from 103 Argentinean bread wheat cultivars. For the Wx-A1 locus, Wx-A1a and a novel molecular allele designed Wx-A1g were detected. Wx-B1 locus showed three alleles (Wx-B1a, Wx-B1b, Wx-B1e), and Wx-D1 locus showed only the Wx-D1a allele. Novel single-locus allele specific markers for Wx-A1b, Wx-B1b and Wx-D1b null alleles were...
Tipo: Journal article Palavras-chave: Characterization; Molecular markers; Triticum aestivum L.; Starch; Waxy genes; Wheat.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100004
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Genetic variability in Brazilian wheat cultivars assessed by microsatellite markers Genet. Mol. Biol.
Schuster,Ivan; Vieira,Elisa Serra Negra; Silva,Glacy Jaqueline da; Franco,Francisco de Assis; Marchioro,Volmir Sérgio.
Wheat (Triticum aestivum) is one of the most important food staples in the south of Brazil. Understanding genetic variability among the assortment of Brazilian wheat is important for breeding. The aim of this work was to molecularly characterize the thirty-six wheat cultivars recommended for various regions of Brazil, and to assess mutual genetic distances, through the use of microsatellite markers. Twenty three polymorphic microsatellite markers (PMM) delineated all 36 of the samples, revealing a total of 74 simple sequence repeat (SSR) alleles, i.e. an average of 3.2 alleles per locus. Polymorphic information content (PIC value) calculated to assess the informativeness of each marker ranged from 0.20 to 0.79, with a mean of 0.49. Genetic distances among...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Germplasm; Cultivar characterization; Cluster analysis; Molecular markers.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300019
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Genetic variability in maize and teosinte populations estimated by microsatellites markers Ciência Rural
Terra,Tatiana de Freitas; Wiethölter,Paula; Almeida,Cícero Carlos de Souza; Silva,Sérgio Delmar dos Anjos e; Bered,Fernanda; Sereno,Maria Jane Cruz de Melo; Barbosa Neto,José Fernandes.
Wild species are important sources of genetic variability and may be exploited by breeding programs. Crosses between teosinte and maize occur freely and teosinte serves as genetic source of agronomic traits for introduction in maize. The objective of this study was to estimate genetic variability among and within maize and teosinte populations (Zea mays mexicana). Two sweet maize populations (BR400 and BR402), two common maize populations (Suwan and Pampa) and one teosinte population were analyzed using microsatellites markers. Results indicated that 64,5% of the variation was detected within the populations, suggesting the possibility of obtaining genetic progress by selection within each population. The analysis with 25 microsatellites loci enabled the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays mays; Zea mays mexicana; Genetic diversity; Flooding tolerance; Molecular markers.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200003
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Genetic variability of Brazilian isolates of Alternaria alternata detected by AFLP and RAPD techniques BJM
Dini-Andreote,Francisco; Pietrobon,Vivian Cristina; Andreote,Fernando Dini; Romão,Aline Silva; Spósito,Marcel Bellato; Araújo,Welington Luiz.
The Alternaria brown spot (ABS) is a disease caused in tangerine plants and its hybrids by the fungus Alternaria alternata f. sp. citri which has been found in Brazil since 2001. Due to the recent occurrence in Brazilian orchards, the epidemiology and genetic variability of this pathogen is still an issue to be addressed. Here it is presented a survey about the genetic variability of this fungus by the characterization of twenty four pathogenic isolates of A. alternata f. sp. citri from citrus plants and four endophytic isolates from mango (one Alternaria tenuissima and three Alternaria arborescens). The application of two molecular markers Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) had revealed the isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternaria brown spot; Molecular markers; Citrus plants; PCA analysis; Genetic variability.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300032
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Genetic variability of hull-less barley accessions based on molecular and quantitative data PAB
Sayd,Ricardo Meneses; Amabile,Renato Fernando; Faleiro,Fabio Gelape; Bellon,Graciele.
The objective of this work was to characterize and quantify the genetic, molecular, and agronomic variability of hull-less barley genotypes, for the selection of parents and identification of genotypes adapted to the irrigated production system in the Brazilian Cerrado. Eighteen hull-less barley accessions were evaluated, and three covered barley accessions served as reference. The characterization was based on 157 RAPD molecular markers and ten agronomic traits. Genetic distance matrices were obtained based on molecular markers and quantitative traits. Graphic grouping and dispersion analyses were performed. Genetic, molecular, and agronomic variability was high among genotypes. Ethiopian accessions were genetically more similar, and the Brazilian ones...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hordeum vulgare; Genetic diversity; Molecular markers; Quantitative traits..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000200160
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Genetic variability of rice recurrent selection populations as affected by male sterility or manual recombination PAB
Pinheiro,Letícia da Silveira; Rangel,Paulo Hideo Nakano; Vianello,Rosana Pereira; Brondani,Claudio.
The objective of this work was to determine the effect of male sterility or manual recombination on genetic variability of rice recurrent selection populations. The populations CNA-IRAT 4, with a gene for male sterility, and CNA 12, which was manually recombined, were evaluated. Genetic variability among selection cycles was estimated using14 simple sequence repeat (SSR) markers. A total of 926 plants were analyzed, including ten genitors and 180 individuals from each of the evaluated cycles (1, 2 and 5) of the population CNA-IRAT 4, and 16 genitors and 180 individuals from each of the cycles (1 and 2) of CNA 12. The analysis allowed the identification of alleles not present among the genitors for both populations, in all cycles, especially for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa; Allele variation; Rice breeding; Molecular markers; Population improvement.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000600011
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Genetic variability of the Brazilian hair sheep breeds PAB
Paiva,Samuel Rezende; Silvério,Vanessa Chaves; Egito,Andréa Alves; McManus,Concepta; Faria,Danielle Assis de; Mariante,Arthur da Silva; Castro,Sílvia Ribeiro; Albuquerque,Maria do Socorro Maués; Dergam,Jorge Abdala.
The objectives of this work were to investigate the genetic structure of the Brazilian hair sheep breeds and to determine the origin of the Santa Inês breed. Molecular similarity was determined using Randomly Amplified Polymorphic DNA - Polymerase Chain Reaction markers in 238 individuals from five naturalized sheep breeds: Santa Inês (48 animals), Rabo Largo (48), Somali (48), Morada Nova (48) and Bergamasca (46), collected in Goiás, Sergipe, Bahia, and Ceará States as well as in the Federal District. Fifty-four loci were selected from 19 primers, after a pilot test using 140 primers. Qualitative analyses indicate diagnostic markers for all breeds. All breeds were significantly different from each other. Interbreed differences were explained by 14.92% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovis aries; Conservation genetics; Domestic animal diversity; Molecular markers; RAPD.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000900008
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Genetic Variability of the Tomato Leaf Miner (Tuta absoluta Meirick; Lepidoptera: Gelechiidae), in Tunisia, Inferred from RAPD-PCR Chilean J. Agric. Res.
Bettaibi,Asma; Mezghani-Khemakhem,Maha; Bouktila,Dhia; Makni,Hanem; Makni,Mohamed.
The tomato leaf miner Tuta absoluta Meyrick has invaded tomato (Solanum lycopersicum L.) crop in Tunisia since 2008 and is representing today a major threat to the production of this crop. In this study, we used the Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technology to assess the genetic variability within and among seven populations of T. absoluta, collected on tomato from different regions in Tunisia. Using five RAPD-PCR primers and 108 individuals, 140 polymorphic fragments were recorded. From 335 different RAPD phenotypes generated, 71 were redundant and 264 unique to a specific population. The genetic structure of T. absoluta was investigated using analysis of molecular variance (AMOVA), genetic distances (Fst) and...
Tipo: Journal article Palavras-chave: Invasive species; Molecular markers; Insect populations; Gene flow.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200008
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Genetics and breeding of sheep in Brazil R. Bras. Zootec.
McManus,Concepta; Paiva,Samuel Rezende; Araújo,Ronyere Olegário de.
Studies in genetics and breeding of sheep in Brazil have increased significantly in recent years. These involve research in characterization, breeding and crossing sheep using new technologies available incorporating both classical quantitative and molecular genetics. Improvements in statistical techniques, computational resources as well as analysis of DNA and gaps in present knowledge and opportunities for possible research are pointed out. There is a need for greater interaction between various groups working in the country as well as interactions with other disciplines such as Geographical Information Systems, Statistics, Bioinformatics, as well as biological studies such as physiology and proteomics.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breed; Disease; Heat tolerance; History; Molecular markers; Ovis aries.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300026
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GenomicLand: Software for genome-wide association studies and genomic prediction Agronomy
Azevedo, Camila Ferreira; Nascimento, Moysés; Fontes, Vitor Cunha; Silva, Fabyano Fonseca e; Resende, Marcos Deon Vilela de; Cruz, Cosme Damião.
 GenomicLand is free software intended for prediction and genomic association studies based on the R software. This computational tool has an intuitive interface and supports large genomic databases, without requiring the user to use the command line. GenomicLand is available in English, can be downloaded from the Internet (https://licaeufv.wordpress.com/), and requires the Windows or Linux operating system. The software includes statistical procedures based on mixed models, Bayesian inference, dimensionality reduction and artificial intelligence. Examples of data files that can be processed by GenomicLand are available. The examples are useful to learn about the operation of the modules and statistical procedures.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Statistical analysis; Genomic analysis; Molecular markers; Biometrics..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/45361
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GenomicLand: Software for genome-wide association studies and genomic prediction Agronomy
Azevedo, Camila Ferreira; Nascimento, Moysés; Fontes, Vitor Cunha; Silva, Fabyano Fonseca e; Resende, Marcos Deon Vilela de; Cruz, Cosme Damião.
 GenomicLand is free software intended for prediction and genomic association studies based on the R software. This computational tool has an intuitive interface and supports large genomic databases, without requiring the user to use the command line. GenomicLand is available in English, can be downloaded from the Internet (https://licaeufv.wordpress.com/), and requires the Windows or Linux operating system. The software includes statistical procedures based on mixed models, Bayesian inference, dimensionality reduction and artificial intelligence. Examples of data files that can be processed by GenomicLand are available. The examples are useful to learn about the operation of the modules and statistical procedures.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Statistical analysis; Genomic analysis; Molecular markers; Biometrics..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/45361
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Heterologous microsatellite primer pairs informative for the whole genus Arachis Genet. Mol. Biol.
Hoshino,Andrea Akemi; Bravo,Juliana Pereira; Angelici,Carla M.L.C.D.; Barbosa,Andréa V. Gobbi; Lopes,Catalina Romero; Gimenes,Marcos A..
The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peanut; Genetic relationship; Molecular markers; Wild relatives.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400016
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HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle R. Bras. Zootec.
Baena,Marielle Moura; Tizioto,Polyana Cristiane; Meirelles,Sarah Laguna Conceição; Regitano,Luciana Correia de Almeida.
ABSTRACT The purpose of this study was to access and characterize single nucleotide polymorphisms (SNP) located within the HSF1 and HSPA6 candidate genes for adaptability in Angus breed raised in subtropical climate. Samples of DNA from 20 animals representing extreme phenotypes for adaptability traits were obtained. Sequence variations in the candidate genes were described by sequencing target regions. We identified 12 SNP located in the HSF1 gene. Moreover, four of the six SNP found in the HSPA6 gene cause amino acid substitutions in protein-coding regions. We also identified a representative SNP (called tag SNP) in a region of the HSF1 gene with high linkage disequilibrium (r2 = 0.87) that may represent 11 SNP located in this gene. Minor allele...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angus; Molecular markers; Thermoregulation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100402
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Huella genética de siete razas de maíz de Valles Altos de México. Colegio de Postgraduados
Rocandio Rodríguez, Mario.
Dentro de la diversidad del maíz (Zea mays L.) de México, uno de los grupos de mayor interés es el del complejo piramidal que se cultiva en Valles Altos centrales de México. Con la finalidad de caracterizar la diversidad genética de siete razas de maiz de Valles Altos, se realizó un estudio utilizando variables morfológicas y marcadores moleculares (SSRs). Se hizo, además, un análisis simultáneo de los datos disponibles sobre dos líneas de evidencia, para hacer clasificaciones más robustas. Se incluyeron entre 8 y 26 accesiones que han sido consideradas por diferentes autores como representativas de las razas Arrocillo Amarillo, Cónicos, Elotes Cónicos, Palomero Toluqueño, Chalqueño y Purépecha. La adquisición de semillas se realizó mediante solicitud a...
Palavras-chave: Zea mays L.; Diversidad genética; Marcadores moleculares; Teocintle; Análisis conjunto; Genetic diversity; Molecular markers; Teosinte; Joint analysis; Genética; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/2092
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Hybrids between Pseudoplatystoma corruscans and P. reticulatum (Siluriformes: Pimelodidae) previously reported in the Upper Paraná River are likely escapes from aquaculture farms: evidence from microsatellite markers Rev. Bras. Zool.
Baggio,Rafael A.; Moretti,Caroline B.; Bialetzki,Andréa; Boeger,Walter A..
ABSTRACT The production of hybrids of the 'pintado', Pseudoplatystoma corruscans (Spix & Agassiz, 1829) and 'cachara', Pseudoplatystoma reticulatum (Eigenmann & Eigenmann, 1889) in captivity has generated many concerns about the possibility of introduction of farmed hybrids into natural environments. In the last decade, hybrids between these species, known as 'pintachara' or 'cachapinta', were reported from different regions of the Upper Paraná River basin. Prospection of these hybrids is important in order to orient conservation programs for the species involved. Knowledge of the presence of these hybrids will direct conservation strategies towards prevention and/or mitigation of the effects of cross breeding in natural populations of P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation genetics; Fish larvae; Interspecific hybrids; Introgression; Molecular markers.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702016000201303
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Identification and characterization of an original grapevine cultivar (Vitis vinifera) found in Chile Ciencia e Investigación Agraria
Gonzalez,Alvaro S; Massera,Franco; Moscoso,Daniel; Hinrichsen,Patricio; Montenegro,Gloria; Laucou,Valerie; Lacombe,Thierry; Boursiquot,Jean-Michel; Pszczólkowski,Philippo.
Currently, many monovarietal vineyards present a minority of mixed cultivars. Some of these cultivars are unknown and could represent an opportunity to discover new and potentially useful genotypes both for research and production purposes. In a 'Carmenère' vineyard planted in 1994 in Palmilla (Colchagua Valley, Chile), a minority presence of other cultivars was found. The present work covers for the first time the identification and characterization of one of these cultivars, which presented a very poor fruit set. Through classic ampelography, it was not possible to associate the studied cultivar with any known cultivar in Chile. However, through a simple sequence repeats (SSR) comparison against the database of the INRA Germplasm Repository "Domaine de...
Tipo: Journal article Palavras-chave: Ampelography; Chile; Coulure; Molecular markers; Poor fruit set; SSR; Triploid.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200016
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